Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade.

Aberrant glycosylation is a crucial strategy employed by cancer cells to evade cellular immunity. However, it's unclear whether homologous recombination (HR) status-dependent glycosylation can be therapeutically explored. Here, we show that the inhibition of branched N-glycans sensitizes HR-proficient, but not HR-deficient, epithelial ovarian cancers (EOCs) to immune checkpoint blockade (ICB). In contrast to fucosylation whose inhibition sensitizes EOCs to anti-PD-L1 immunotherapy regardless of HR-status, we observe an enrichment of branched N-glycans on HR-proficient compared to HR-deficient EOCs. Mechanistically, BRCA1/2 transcriptionally promotes the expression of MGAT5, the enzyme responsible for catalyzing branched N-glycans. The branched N-glycans on HR-proficient tumors augment their resistance to anti-PD-L1 by enhancing its binding with PD-1 on CD8+ T cells. In orthotopic, syngeneic EOC models in female mice, inhibiting branched N-glycans using 2-Deoxy-D-glucose sensitizes HR-proficient, but not HR-deficient EOCs, to anti-PD-L1. These findings indicate branched N-glycans as promising therapeutic targets whose inhibition sensitizes HR-proficient EOCs to ICB by overcoming immune evasion.

Association between the remnant cholesterol to high-density lipoprotein cholesterol ratio and the risk of coronary artery disease.

BACKGROUND: The purpose of this study was to evaluate the relationship between remnant cholesterol (RC) to high-density lipoprotein cholesterol (HDL-C) ratio and the risk of coronary artery disease (CAD). We also investigated the clinical value of RC/HDL-C ratio in evaluating the severity of CAD and in predicting the short-term prognosis of CAD patients. METHODS: In total, 615 patients were enrolled and they were classified into a CAD group (418 cases) and a normal group (197 cases) according to the results of coronary angiography. Serum RC/HDL-C ratio and Gensini score were calculated. Multivariate logistic regression and receiver operating characteristic (ROC) curves were employed to evaluate the association between RC/HDL-C ratio and CAD. The effect of RC/HDL-C ratio on the progression of major adverse cardiovascular events (MACEs) was also explored. RESULTS: Increased RC/HDL-C ratio was associated with an increased risk of CAD (OR: 11.122; 95% CI: 5.903-20.954; P  < 0.001). When stratified by CAD subtypes, increased RC/HDL-C ratio was correlated with a greater risk of acute coronary syndrome (ACS) (OR:1.549; 95% CI: 1.014-2.364; P  < 0.05). Compared with the first quartile, the 4th quartile of RC/HDL-C ratio had a 9.774-fold ( P  = 0.000) increase in the odds ratio for CAD, and a 2.241-fold ( P  = 0.017) increase in the odds ratio for ACS. RC/HDL-C ratio was an independent determinant of Gensini score ≥32 (OR: 2.138, 95% CI:1.389-3.292, P  < 0.01), and multi-branch (MVD) (OR: 2.245; 95% CI: 1.468-3.443; P  < 0.001). The prevalence of Gensini score ≥32 and MVD in the 4th quartile of RC/HDL-C ratio group were much higher than that of other quartile groups ( P  < 0.01). Moreover, the areas under the ROC for the predictive value of RC/HDL-C ratio for CAD, ACS, Gensini score ≥32, and MVD were 0.702, 0.563, 0.602, and 0.669, respectively. Furthermore, the incidence of MACEs was significantly increased in CAD patients with levels of RC/HDL-C ratio ( P  < 0.05). CONCLUSION: RC/HDL-C ratio plays an important role in the progression and severity of CAD.

TXNRD1 drives the innate immune response in senescent cells with implications for age-associated inflammation.

Sterile inflammation, also known as 'inflammaging', is a hallmark of tissue aging. Cellular senescence contributes to tissue aging, in part, through the secretion of proinflammatory factors collectively known as the senescence-associated secretory phenotype (SASP). The genetic variability of thioredoxin reductase 1 (TXNRD1) is associated with aging and age-associated phenotypes such as late-life survival, activity of daily living and physical performance in old age. TXNRD1's role in regulating tissue aging has been attributed to its enzymatic role in cellular redox regulation. Here, we show that TXNRD1 drives the SASP and inflammaging through the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) innate immune response pathway independently of its enzymatic activity. TXNRD1 localizes to cytoplasmic chromatin fragments and interacts with cGAS in a senescence-status-dependent manner, which is necessary for the SASP. TXNRD1 enhances the enzymatic activity of cGAS. TXNRD1 is required for both the tumor-promoting and immune surveillance functions of senescent cells, which are mediated by the SASP in vivo in mouse models. Treatment of aged mice with a TXNRD1 inhibitor that disrupts its interaction with cGAS, but not with an inhibitor of its enzymatic activity alone, downregulated markers of inflammaging in several tissues. In summary, our results show that TXNRD1 promotes the SASP through the innate immune response, with implications for inflammaging. This suggests that the TXNRD1-cGAS interaction is a relevant target for selectively suppressing inflammaging.

Two-Dimensional Van Der Waals Thin Film and Device.

In the rapidly evolving field of thin-film electronics, the emergence of large-area flexible and wearable devices has been a significant milestone. Although organic semiconductor thin films, which can be manufactured through solution processing, have been identified, their utility is often undermined by their poor stability and low carrier mobility under ambient conditions. However, inorganic nanomaterials can be solution-processed and demonstrate outstanding intrinsic properties and structural stability. In particular, a series of two-dimensional (2D) nanosheet/nanoparticle materials have been shown to form stable colloids in their respective solvents. However, the integration of these 2D nanomaterials into continuous large-area thin with precise control of layer thickness and lattice orientation still remains a significant challenge. This review paper undertakes a detailed analysis of van der Waals thin films, derived from 2D materials, in the advancement of thin-film electronics and optoelectronic devices. The superior intrinsic properties and structural stability of inorganic nanomaterials are highlighted, which can be solution-processed and underscor the importance of solution-based processing, establishing it as a cornerstone strategy for scalable electronic and optoelectronic applications. A comprehensive exploration of the challenges and opportunities associated with the utilization of 2D materials for the next generation of thin-film electronics and optoelectronic devices is presented.

Black-Blood Magnetization Prepared 2 Rapid Acquisition Gradient Echoes: A Fast and Three-Dimensional MR Black-Blood T1 Mapping Technique for Quantitative Assessment of Atherosclerosis and Venous Thrombosis.

BACKGROUND: Blood flow signals may be a confounder in quantifying T1 values of plaque or thrombus and how to realize black-blood T1 mapping remains a challenge task. PURPOSE: To develop a fast and three-dimensional black-blood T1 mapping technique for quantitative assessment of atherosclerosis and venous thrombosis. STUDY TYPE: Sequence development and optimization via phantoms and volunteers as well as pilot prospective. PHANTOM AND SUBJECTS: Numerical simulations, a standard phantom, 8 healthy volunteers (mean age, 22 ± 1 years; 5 males), and 19 patients (mean age, 57 ± 14 years; 13 males) with atherosclerosis or venous thrombosis. FIELD STRENGTH/SEQUENCE: 3T/inversion recovery spin-echo sequence (IR-SE), magnetization prepared 2 rapid acquisition gradient echoes (MP2RAGE), and black-blood prepared MP2RAGE (BB-MP2RAGE). ASSESSMENT: The black-blood preparation (i.e., delay alternating with nutation for tailored excitation, DANTE) was incorporated into MP2RAGE for black-blood T1 mapping. The BB-MP2RAGE was optimized numerically based on the Bloch equation, and then the phantom study was performed to verify the accuracy of T1 mapping by BB-MP2RAGE against IR-SE and MP2RAGE. Preliminary clinical validation was prospectively performed to assess the flow suppression effect and its potential application in plaque and thrombosis identification. STATISTICAL TESTS: Pearson correlation test, Bland-Altman analysis, paired t-test, and intraclass correlation coefficient. A P value <0.05 indicates a statistically significant difference. RESULTS: Phantom experiments showed comparable accuracy of T1 maps by BB-MP2RAGE with IR-SE and MP2RAGE (all r2 > 0.99); Compared to MP2RAGE, BB-MP2RAGE effectively nulled the blood flow signals, and had a significant improvement in contrast-to-noise ratio between static tissue and blood (250.5 ± 66.6 vs. 91.9 ± 35.9). BB-MP2RAGE can quantify plaque or thrombus T1 relaxation time with blood flow signal suppression. DATA CONCLUSION: Accurate T1 mapping with sufficient blood flow suppression was achieved by BB-MP2RAGE. BB-MP2RAGE has the potential to quantitatively characterize atherosclerosis and venous thrombosis. LEVEL OF EVIDENCE: 1 TECHNICAL EFFICACY: Stage 1.

Impact of bronchoscopic thermal vapor ablation on lung volume reduction in patients with emphysema: a meta-analysis.

BACKGROUND: Bronchoscopic lung volume reduction (LVR) could significantly improve pulmonary function and quality of life in patients with emphysema. We aimed to assess the efficacy and safety of bronchoscopic thermal vapor ablation (BTVA) on LVR in patients with emphysema at different stage. METHODS: A systematic search of database including PubMed, Embase and Cochrane library was conducted to determine all the studies about bronchoscopic thermal vapor ablation published through Dec 1, 2022. Related searching terms were "lung volume reduction", "bronchoscopic thermal vapor ablation", "bronchial thermal vapor ablation" "BTVA" and "emphysema", "efficacy" and"safety". We used standardized mean difference (SMD) to analyze the summary estimates for BTVA therapy. RESULTS: We retrieved 30 records through database search, and 4 trials were selected for meta-analysis, including 112 patients with emphysema. Meta-analysis of the pooled effect showed that levels of forced expiratory volume in 1 s (FEV1), residual volume (RV), total lung capacity (TLC), 6-min walk distance (6MWD) and St George's Respiratory Questionnaire (SGRQ) were significantly improved in patients with emphysema following BTVA treatment between 6 months vs. baseline. Additionally, no significant changes in FEV1, RV, TLC and SGRQ occurred from 3 to 6 months of follow-up except for 6MWD. The magnitude of benefit was higher at 3 months compared to 6 months. The most common complications at 6 months were treatment-related chronic obstructive pulmonary disease (COPD) exacerbations (RR: 12.49; 95% CI: 3.06 to 50.99; p < 0.001) and pneumonia (RR: 9.49; 95% CI: 2.27 to 39.69; p < 0.001). CONCLUSIONS: Our meta-analysis provided clinically relevant information about the impact and safety of BTVA on predominantly upper lobe emphysema. Particularly, short-term significant improvement of lung function and quality of life occurred especially within the initial 3 months. Further large-scale, well-designed long-term interventional investigations are needed to clarify this issue.

Resilience mediates the effect of peer victimization on quality of life in Chongqing adolescents: from a perspective of positive childhood experiences.

Background: Peer victimization is a harmful experience that contributed to one's psychological problems, physical health deterioration, and so on. Quality of life (QoL) is an important indicator of adolescent health assessment. To identify potential pathways of positive experiences in preventing peer victimization's detrimental effects and then provide intervention ideas for adolescent health, this study was conducted to examine the relationship between peer victimization and QoL in Chongqing adolescents and discover whether resilience plays a mediating role and positive childhood experiences (PCEs) act as a moderating role in the relationship. Methods: Data were the first follow-up of a cohort study conducted in four complete middle schools in two districts of Chongqing, China. Self-designed peer victimization items, the Connor-Davidson Resilience Scale, the Adolescent Quality of Life Scale, and the Benevolent Childhood Experiences Scale were used. We investigated the differences and correlations in peer victimization, QoL, and resilience between the two PCEs groups. Mplus version 8.3 was used to analyze the mediating role of resilience and the moderating role of PCEs in peer victimization and QoL. Results: Peer victimization, resilience, and QoL differed between the two PCEs groups (P < 0.001). Peer victimization negatively correlated with QoL and resilience, while resilience positively correlated with QoL (P < 0.001). In the models with total QOL as the dependent variable, the indirect effect was -0.431 (8.08% of the total effect) in the low-PCEs group vs. -2.077 (41.97% of the total effect) in the high-PCEs group. In the models with four dimensions of QOL as the dependent variable, the indirect effects ranged from -0.054 to -0.180 (6.07-12.95% of the total effects) in the low-PCEs group and from 0.295 to -0.823 in the high-PCEs group (35.89-68.76% of the total effects). Both total and indirect effects were significant (P < 0.05). In addition, the differences in indirect effects were significant between the two PCEs groups (P < 0.05), while differences in total and direct effects were almost not apparent. Conclusion: Resilience partially mediated the effect of peer victimization on QoL in Chongqing adolescents, and PCEs moderated this mediation. Schools, families, and society should focus on resilience intervention and prioritize the enhancement of PCEs for improving adolescent QoL.

Synthesis of 1,3,5-triphenyl-1,2,4-triazole derivatives and their neuroprotection by anti-oxidative stress and anti-inflammation and protecting BBB.

Acute ischemic stroke (AIS) is a serious cardiovascular and cerebrovascular disease; Oxidative stress and neuroinflammation are important factors which destroy blood-brain barrier (BBB) in AIS. In the study, a series of 1,3,5-triphenyl-1,2,4-triazole derivatives were designed and synthesized; the optimal compound 9 was obtained by screening their anti-oxidant and anti-inflammatory effects; the neuroprotection effect of compound 9 was evaluated with a rat middle cerebral artery occlusion (MCAO) model. Subsequently, the mechanism of neuroprotection were explored via Western blot. The results prompt compound 9 maybe exert anti-AIS neuroprotection by inhibiting oxidative stress and neuroinflammation inhibition by inhibiting Keap1, COX-2 and iNOS. At the same time, it can protect BBB by reducing glycocalyx degradation and matrix metallopeptidase-9 levels. Its LD50 > 1000 mg/kg on mice and hERG channel inhibition IC50 > 30 µM, which lower acute toxicity and hERG channel inhibition would make compound 9 a promising stroke treatment candidate.

The complete mitochondrial genome of the Anas platyrhynchos Linnaeus, 1758 breed Longshengcui and its phylogenetic analyses.

Duck breed Longshengcui (Anas platyrhynchos Linnaeus, 1758 breed Longshengcui, LSC) is one of the famous native breed of the Guangxi Zhuang Nationality Autonomous Region in China. In this study, we report the complete mitochondrial genome of LSC. The mitogenome (GenBank accession no. MZ895120) has 16,602 bp in length and consisted of the well-known 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and the control region. The phylogenetic analysis showed that LSC and Zhijiang duck have highly similar genetic relationship. These results are helpful for the conservation of genetic resources and phylogeny of this species.

Electrochemical Decalcification-Exfoliation of Two-Dimensional Siligene, SixGey: Material Characterization and Perspectives for Lithium-Ion Storage.

A two-dimensional (2D) silicene-germanene alloy, siligene (SixGey), a single-phase material, has attracted increased attention due to its two-elemental low-buckled composition and unique physics and chemistry. This 2D material has the potential to address the challenges caused by low electrical conductivity and the environmental instability of corresponding monolayers. Yet, the siligene structure was studied in theory, demonstrating the material's great electrochemical potential for energy storage applications. The synthesis of free-standing siligene remains challenging and therefore hinders the research and its application. Herein we demonstrate nonaqueous electrochemical exfoliation of a few-layer siligene from a Ca1.0Si1.0Ge1.0 Zintl phase precursor. The procedure was conducted in an oxygen-free environment applying a -3.8 V potential. The obtained siligene exhibits a high quality, high uniformity, and excellent crystallinity; the individual flake is within the micrometer lateral size. The 2D SixGey was further explored as an anode material for lithium-ion storage. Two types of anode have been fabricated and integrated into lithium-ion battery cells, namely, (1) siligene-graphene oxide sponges and (2) siligene-multiwalled carbon nanotubes. The as-fabricated batteries both with/without siligene exhibit similar behavior; however there is an increase in the electrochemical characteristics of SiGe-integrated batteries by 10%. The corresponding batteries exhibit a 1145.0 mAh·g-1 specific capacity at 0.1 A·g-1. The SiGe-integrated batteries demonstrate a very low polarization, confirmed by their good stability after 50 working cycles and a decrease in the solid electrolyte interphase level that occurs after the first discharge/charge cycle. We anticipate the growing potential of emerging two-component 2D materials and their great promise for energy storage and beyond.

One-Step Electrochemical Synthesis of AlO x -Passivated Twisted-Phosphorene Nanosheets for Potentially Stable Energy Storage Devices.

Black phosphorus (BP), a promising 2D material for electronics, energy storage, catalysis, and sensing, has sparked a research boom. However, exfoliated thin-layered BP is unstable and can easily be degraded under environmental conditions, severely limiting its practical applications. In this context, a simple and cost-effective method has been proposed that involves electrochemically exfoliating BP and simultaneously electrochemically depositing aluminum oxide (AlO x ) for passivation of the exfoliated BP. The ambient stability of the exfoliated BP is studied using a time-dependent atomic force microscope (AFM). The AlO x capping layer significantly improves the environmental stability of BP compared to uncapped BP. The thermal stability of the resulting BP is evaluated using power-dependent Raman spectroscopy. The results show that the AlO x -passivated BP has increased thermal stability, with only a slight shift in peak position toward higher Raman power intensity. These properties can make the material suitable for stable energy storage devices. Interestingly, the electrochemical exfoliation and passivation processes resulted in the BP with a twist angle (9.86°), which is expected to exhibit unique electronic properties similar to those of graphene with a twist angle.

Targeting the mevalonate pathway suppresses ARID1A-inactivated cancers by promoting pyroptosis.

ARID1A, encoding a subunit of the SWI/SNF complex, is mutated in ∼50% of clear cell ovarian carcinoma (OCCC) cases. Here we show that inhibition of the mevalonate pathway synergizes with immune checkpoint blockade (ICB) by driving inflammasome-regulated immunomodulating pyroptosis in ARID1A-inactivated OCCCs. SWI/SNF inactivation downregulates the rate-limiting enzymes in the mevalonate pathway such as HMGCR and HMGCS1, which creates a dependence on the residual activity of the pathway in ARID1A-inactivated cells. Inhibitors of the mevalonate pathway such as simvastatin suppresses the growth of ARID1A mutant, but not wild-type, OCCCs. In addition, simvastatin synergizes with anti-PD-L1 antibody in a genetic OCCC mouse model driven by conditional Arid1a inactivation and in a humanized immunocompetent ARID1A mutant patient-derived OCCC mouse model. Our data indicate that inhibition of the mevalonate pathway simultaneously suppresses tumor cell growth and boosts antitumor immunity by promoting pyroptosis, which synergizes with ICB in suppressing ARID1A-mutated cancers.

The relationship between health literacy and internet addiction among middle school students in Chongqing, China: A cross-sectional survey study.

BACKGROUND: Internet addiction has emerged as a major global concern as a potential adverse impact of internet exposure on adolescents. Internet addiction is associated with many demographic variables; however, there is a lack of consensus on its relationship with health literacy. Therefore, the aim of the present study was to assess the rates of internet addiction and health literacy level among middle school students (grades 7 to 12) in Chongqing, China, as well as to investigate the association between them. METHODS: A cross-sectional, questionnaire-based study was conducted among 8971 students who were randomly selected by using stratified cluster sampling between November and December 2019. The Internet Addiction Diagnostic Questionnaire, Adolescent Health Literacy Scale and a self-designed basic information questionnaire were used to collect data. Chi-square tests were performed to compare the differences in the distribution of internet addiction across health literacy levels as well as some sociodemographic characteristics. Multivariate logistic regression analyses were conducted to identify the association between health literacy and internet addiction. RESULTS: The prevalence of internet addiction among middle school students in Chongqing was 6.1%. The percentage of the students who spent more than 4 hours online every day in the past week was 14.3%. In addition, 26.7%, 26.0%, 28.3% and 26.3% of the participants reported low functional, interactive, critical and total health literacy, respectively. After adjusting for the confounding effects of demographics, multivariate regression analysis showed that critical health literacy was a protective variable for internet addiction, while functional, and interactive health literacy were the risk variable (P<0.05). Furthermore, the internet addiction rates were higher among boys, students with good peer relationships, and students without parental supervision, whereas internet addiction rates were lowest among students in grade 12. CONCLUSIONS: The prevalence of internet addiction among middle school students in Chongqing is relatively high. Internet addiction is strongly negatively associated with critical health literacy, but it is positively associated with functional and interactive health literacy but not total health literacy. This study provides preliminary evidence for the predictive role of health literacy in internet addiction of adolescents.

The simulation, regulation capacity assessment and coping strategy of rainstorm runoff waterlogging in Zhu pai-chong Basin of Nanning, China.

Currently, China is experiencing a phase of rapid urbanization. With the frequent occurrence of extreme rainfall events within the context of climate change, the problem of heavy rainfall and waterlogging in many cities is very prominent. In November 2020, China issued a proposal for the construction of sponge cities across the entire region to significantly enhance the rainfall flood prevention and drainage capacity of cities and effectively improve the resilience of sponge city systems for flooding management. Therefore, this paper selected the Zhu pai-chong watershed in Nanning with frequent waterlogging disasters as an example. Based on underlying surface information, We used a coupled SWMM-LISFOOD model to simulate runoff and waterlogging processes and analyze the spatial and temporal evolution characteristics of the basin under 10 designed rainstorm return periods (0.25a-50a). The results confirm the substantial spatial and temporal variabilities of the runoff coefficient in the study area; impermeability was the main factor contributing to high runoff coefficient values. The spatial distribution characteristics of inundation area was general dispersion and local linear aggregation. Furthermore, this study assessed the effect of the control rate of blue‒green‒gray facilities on the actual storms, and the value ranged from only 48.6% (0.25a)-24.05% (50a). This study quantified the two-dimensional distribution of rainfall storage volume thresholds with or without considering the discharged from the pipe network. Quantitative mapping between the elements of "rainfall-storage volume of blue‒green‒gray facilities-runoff-drainage capacity of the pipe network-waterlogging level" was conducted within the study area as an example. Finally, an overall technical process scheme for rainfall and waterlogging management was proposed. The scheme covered the hydrological‒hydraulic mechanism, storage function of sponge facilities, engineering control response, nonengineering measures and intelligent management of rainfall and waterlogging during sponge city construction, which could provide critical scientific support for effective promotion of the construction of sponge cities in China.

Cluster Analysis of Sleep Time and Adolescent Health Risk Behaviors.

Adolescents are prone to multiple health risk behaviors. These might lead to insufficient sleep, which is inconducive to adolescent growth. Therefore, this study explored the impact of a cluster of adolescent health risk behaviors on sleep time, providing a reference for designing relevant intervention measures. From November to December 2019, a stratified cluster sampling method was used to sample middle and high schools in 4 functional districts of Chongqing, China. A total of 8546 participants were selected for a questionnaire survey. Two-step clustering helped identify the health risk behavior clusters. Multivariate logistic regression models helped examine the association between the different clusters and sleep time. The rate of insufficient sleep was 65.8%. Three types of clusters were identified, namely (1) high-risk (poor) cluster (17.3%), (2) low physical activity (medium) cluster (55.1%), and (3) low-risk (good) cluster (27.6%). The high-risk and low physical activity clusters showed that the adjusted OR values of 1.471 (1.266-1.710) and 1.174 (1.052-1.310) were significantly associated with insufficient sleep (P < .001). Adolescent health risk behaviors were clustered, and different clusters had different sleep time. Schools authorities and healthcare practitioners should formulate effective intervention measures according to the characteristics of different clusters to promote healthy growth among adolescents.

Prediction of short-term treatment outcome of nasopharyngeal carcinoma based on voxel incoherent motion imaging and arterial spin labeling quantitative parameters.

Purpose: To evaluate the early response of chemoradiotherapy (CRT) in nasopharyngeal carcinoma (NPC) based on intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) and three-dimensional pseudo-continuous arterial spin labeling (3D pCASL). Materials and methods: Forty patients diagnosed with NPC were recruited and divided into complete remission (CR) and partial remission (PR) group after CRT. All patients underwent IVIM and ASL and the related parameters was obtained. These parameters include pure diffusion coefficient (D), pseudo-diffusion coefficient (D*), perfusion fraction (f), average blood flow ( BFavg), minimum blood flow (BFmin), and maximum blood flow (BFmax). Student's t test was used to compare the difference in ASL and IVIM derived parameters between CR and PR. The Areas under curve (AUC) of the receiver operating characteristic (ROC) was used to analyze the diagnostic performance of each parameter of ASL and IVIM to the treatment outcome. Results: the D value of IVIM in CR group was lower than that of the PR group ( P = 0.014),. Among the parameters of ASL, the BFavg and BFmax of the CR group were higher than those of the PR group(p = 0.004,0.013), but the BFmin had no statistical significance in the two groups（P = 0.54）. AUC of D, BFavg, and BFmax is about 0.731, 0.753, and 0.724, respectively, all of their combined AUC diagnosis was 0.812. Conclusion: The early response of NPC after CRT can predict by IVIM's diffusion parameters and ASL-related blood flow parameters.

Dedicated training of explosive strength in the abdominal core of soccer players / Treinamento dedicado da força explosiva no core abdominal dos jogadores de futebol / Entrenamiento específico de la fuerza explosiva en el core abdominal de futbolistas

ABSTRACT Introduction: Physical confrontation in soccer games presents greater demands on athletes' physical fitness. A soccer player's speed, strength, flexibility, coordination, endurance, and explosiveness can affect the body's fighting capacity. Objective: This study analyzes the relationship between strength training in soccer players' abdominal core and physical fitness. Methods: The effect of abdominal core stability strength training on performance improvement in volunteer soccer players selected as research subjects was verified by random division into two groups (experimental and control groups). Both groups performed daily training. The experimental group added special abdominal core training. Mathematical-statistical algorithms were used to statistically analyze the physical indicators of the two groups of volunteers. Results: The indices of physical fitness and body explosiveness of the two groups of athletes were significantly improved (P<0.05). After systematic training, the competition performance of the experimental group and the physiological and biochemical indicators were better than the control group (P<0.05). Conclusion: After abdominal core training, soccer players' physical fitness and explosive power indexes were improved. Research shows that strength training can help improve abdominal core performance in soccer players. It is recommended that coaches implement abdominal core strength training in the daily training of athletes. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O confronto físico nos jogos de futebol apresenta maiores exigências quanto à aptidão física dos atletas. A velocidade, força, flexibilidade, coordenação, resistência e explosividade de um jogador de futebol podem afetar a capacidade de combate corporal. Objetivo: Este estudo visa analisar a relação entre o treinamento de força no core abdominal dos jogadores de futebol e sua aptidão física. Métodos: O efeito do treinamento de força da estabilidade do core abdominal sobre a melhoria do desempenho nos jogadores de futebol voluntários selecionados como objetos de pesquisa foi verificado por divisão aleatória em dois grupos (grupos experimentais e grupos de controle). Ambos os grupos realizavam treinamentos diários. O grupo experimental acrescentou treinamento especial de core abdominal. Foram utilizados algoritmos matemáticos-estatísticos para analisar os indicadores físicos dos dois grupos de voluntários de forma estatística. Resultados: Os índices de aptidão física e explosividade corporal dos dois grupos de atletas foram significativamente melhorados (P<0,05). Após o treinamento sistemático, o desempenho de competição do grupo experimental e os indicadores fisiológicos e bioquímicos foram melhores que os do grupo de controle (P<0,05). Conclusão: Após o treinamento do core abdominal, a aptidão física dos jogadores de futebol e os índices de potência explosiva foram melhorados. Pesquisas mostram que o treinamento da força pode ajudar a melhorar o desempenho do core abdominal nos jogadores de futebol. Recomenda-se aos treinadores a implementação do treino de força no core abdominal ao treinamento diário dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El enfrentamiento físico en los partidos de fútbol presenta mayores exigencias a la aptitud física de los atletas. La velocidad, la fuerza, la flexibilidad, la coordinación, la resistencia y la explosividad de un jugador de fútbol pueden afectar a la capacidad de combate del cuerpo. Objetivo: Este estudio tiene como objetivo analizar la relación entre el entrenamiento de la fuerza en el core de los jugadores de fútbol y su condición física. Métodos: El efecto del entrenamiento de la fuerza de la estabilidad del core abdominal sobre la mejora del rendimiento en jugadores de fútbol voluntarios seleccionados como sujetos de la investigación se verificó mediante la división aleatoria en dos grupos (grupo experimental y grupo de control). Ambos grupos realizaron un entrenamiento diario. El grupo experimental añadió un entrenamiento especial del core abdominal. Se utilizaron algoritmos matemático-estadísticos para analizar de forma estadística los indicadores físicos de los dos grupos de voluntarios. Resultados: Los índices de aptitud física y explosividad corporal de los dos grupos de atletas mejoraron significativamente (P<0,05). Tras el entrenamiento sistemático, el rendimiento en competición del grupo experimental y los indicadores fisiológicos y bioquímicos fueron mejores que los del grupo de control (P<0,05). Conclusión: Tras el entrenamiento del core abdominal, mejoraron la condición física y los índices de potencia explosiva de los futbolistas. Las investigaciones demuestran que el entrenamiento de fuerza puede ayudar a mejorar el rendimiento del core abdominal en los jugadores de fútbol. Se recomienda que los entrenadores implementen el entrenamiento de fuerza en el core abdominal al entrenamiento diario de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Impacts of suspension training on the physical fitness of swimmers / Impactos do treinamento de suspensão sobre a aptidão física de nadadores / Efectos del entrenamiento en suspensión en la aptitud física de los nadadores

ABSTRACT Introduction Suspension training develops the physical fitness of a swimmer and improves his or her abilities. The specific fitness of a swimmer is the body's ability to adapt to load in swimming. This is also a comprehensive reflection of their physical function, conditioning, athletic ability, and overall health. Objective This study aimed to analyze the effect of suspension training on swimmers' balance, abdominal center strength, and athletic performance. Methods This paper selects several swimmers as research volunteers. They were randomly divided into experimental and control groups. Both of them underwent physical training for three months. The experimental group adopted the suspension training method. The control group used traditional training methods. Mathematical statistics performed data analysis in both groups. Results The physical fitness of the two groups of swimmers was improved substantially after the experiment (P<0.05). The strength balance ability of the experimental group showed better performance(P<0.05). There was a significant difference between the experimental and control groups in the results of fitness index tests (P<0.01). Conclusion Suspension training has a prominent effect on the physical development of swimmers. This modality proved a better efficacy on swimmers' performance. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução O treinamento em suspensão desenvolve a aptidão física de um nadador e aprimora suas habilidades. A aptidão física específica de um nadador é a capacidade corporal de adaptação à carga na natação. Isto é também um reflexo abrangente de sua função física, condicionamento, habilidade atlética e saúde geral. Objetivo Este estudo teve como objetivo analisar o efeito do treinamento em suspensão no equilíbrio da força do centro abdominal e no desempenho atlético dos nadadores. Métodos Este artigo seleciona vários nadadores como voluntários de pesquisa. Eles foram divididos aleatoriamente em grupo experimental e grupo de controle. Ambos foram submetidos a treinamento físico durante três meses. O grupo experimental adotou o método de treinamento em suspensão. O grupo de controle utilizou métodos de treinamento tradicionais. A análise dos dados nos dois grupos foi executada por estatísticas matemáticas. Resultados A aptidão física dos dois grupos de nadadores foi aprimorada substancialmente após o experimento (P<0,05). A capacidade de equilíbrio de força do grupo experimental demonstrou um melhor desempenho(P<0,05). Houve uma diferença significativa entre os grupos experimental e de controle nos resultados dos testes de índice de aptidão física (P<0,01). Conclusão O treinamento de suspensão tem um efeito destacado sobre o desenvolvimento físico dos nadadores. Esta modalidade comprovou uma melhor eficácia sobre o desempenho dos nadadores.Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción El entrenamiento en suspensión desarrolla la aptitud física de un nadador y mejora sus habilidades. La condición física específica de un nadador es la capacidad del cuerpo para adaptarse a la carga en la natación. También es un reflejo exhaustivo de su función física, su acondicionamiento, su capacidad atlética y su salud en general. Objetivo Este estudio tiene como objetivo analizar el efecto del entrenamiento en suspensión sobre el equilibrio de la fuerza del centro abdominal y el rendimiento deportivo de los nadadores. Métodos Este trabajo selecciona a varios nadadores como voluntarios para la investigación. Se dividieron aleatoriamente en grupo experimental y grupo de control. Ambos fueron sometidos a un entrenamiento físico durante tres meses. El grupo experimental adoptó el método de entrenamiento en suspensión. El grupo de control utilizó métodos de entrenamiento tradicionales. El análisis de los datos en ambos grupos se realizó mediante estadística matemática. Resultados La aptitud física de los dos grupos de nadadores mejoró sustancialmente después del experimento (P<0,05). La capacidad de equilibrio de la fuerza del grupo experimental mostró un mejor rendimiento (P<0,05). Hubo una diferencia significativa entre los grupos experimental y de control en los resultados de las pruebas del índice de aptitud física (P<0,01). Conclusión El entrenamiento en suspensión tiene un efecto destacado en el desarrollo físico de los nadadores. Esta modalidad demostró una mayor eficacia en el rendimiento de los nadadores. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

A potent PGK1 antagonist reveals PGK1 regulates the production of IL-1ß and IL-6.

Glycolytic metabolism enzymes have been implicated in the immunometabolism field through changes in metabolic status. PGK1 is a catalytic enzyme in the glycolytic pathway. Here, we set up a high-throughput screen platform to identify PGK1 inhibitors. DC-PGKI is an ATP-competitive inhibitor of PGK1 with an affinity of K d = 99.08 nmol/L. DC-PGKI stabilizes PGK1 in vitro and in vivo, and suppresses both glycolytic activity and the kinase function of PGK1. In addition, DC-PGKI unveils that PGK1 regulates production of IL-1ß and IL-6 in LPS-stimulated macrophages. Mechanistically, inhibition of PGK1 with DC-PGKI results in NRF2 (nuclear factor-erythroid factor 2-related factor 2, NFE2L2) accumulation, then NRF2 translocates to the nucleus and binds to the proximity region of Il-1ß and Il-6 genes, and inhibits LPS-induced expression of these genes. DC-PGKI ameliorates colitis in the dextran sulfate sodium (DSS)-induced colitis mouse model. These data support PGK1 as a regulator of macrophages and suggest potential utility of PGK1 inhibitors in the treatment of inflammatory bowel disease.

Associations between Greenspaces and Individual Health: A Longitudinal Study in China.

Using a longitudinal survey in China, this study identifies the effect of greenspaces on individual health in the aspects of self-rated health, mental health, feeling physical discomfort, and being hospitalized. The normalized difference vegetation index (NDVI) is used to measure the greenery cover of each city, and findings show that higher NDVI leads to the improvement of personal self-rated health status, and it also decreases the probability of being hospitalized, having symptoms of physical discomfort, and being lost in bad mood. The positive health effect of NDVI in the city is much more significant and larger for the middle-aged, the elderly, and the low-educated. The evidence of the three possible channels through which the NDVI of each city shows a positive correlation with individual physical and mental health is found. The increased NDVI in the city encourages residents to foster healthier habits (e.g., decreasing smoking or drinking, increasing sleeping hours), improves air quality and reduces air pollution in each city, and promotes the social cohesion of people, and so the health status of residents is enhanced. This study implies that increasing greenspaces in the city is an effective strategy to improve social welfare and residents' health.